Hotspot

trnK-UUU-trnT-UGU

LSC · 4232-4923 · 692 bp

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Pi 0.0189
MarkerSeek score 68.00
Primer available yes
Label trnK-UUU-trnT-UGU

Feature Metadata

label_name
trnK-UUU-trnT-UGU
feature_type
igs
region
LSC
length_bp
692
variable_sites
28
pi
0.0189
unique_haplotype_count
5
primer_available
yes
alignment_reliability
1.00
markerseek_score
68.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnK-UUU-trnT-UGU_p1 TCTAGCCGCACTTAAAAGCC GCGATGCTCTAACCTCTGAG 50.0 55.0 58.3 57.9 689-844 bp 1.0 83.7
trnK-UUU-trnT-UGU_p2 TCTAGCCGCACTTAAAAGCC GGAATCGAACCGATGACCAT 50.0 50.0 58.3 57.8 721-876 bp 1.0 82.7
trnK-UUU-trnT-UGU_p3 TACCGTTGAGTTAGCAACCC GCGATGCTCTAACCTCTGAG 50.0 55.0 57.5 57.9 661-816 bp 1.0 82.3
trnK-UUU-trnT-UGU_p4 TACCGTTGAGTTAGCAACCC GGAATCGAACCGATGACCAT 50.0 50.0 57.5 57.8 693-848 bp 1.0 81.3
trnK-UUU-trnT-UGU_p5 CGAATCCGTTGCTTCATCCA GCGATGCTCTAACCTCTGAG 50.0 55.0 58.6 57.9 723-878 bp 1.0 80.7