Hotspot

rpl16

LSC · 82655-84160 · 1506 bp

Back to Adinandra

Pi 0.00404

MarkerSeek score 50.10

Primer available yes

Label rpl16

Feature Metadata

label_name: rpl16
feature_type: gene
region: LSC
length_bp: 1506
variable_sites: 9
pi: 0.00404
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 50.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl16_p1	TCCCAAAACCTATTTCCTTTGGT	GGGTTGAATAAAAATTCGATTGACC	39.1	36.0	58.2	57.5	1559-1579 bp	1.0	41.5
rpl16_p2	CCCAAAACCTATTTCCTTTGGT	GGGTTGAATAAAAATTCGATTGACC	40.9	36.0	56.8	57.5	1558-1578 bp	1.0	41.5
rpl16_p3	TCCCAAAACCTATTTCCTTTGGT	AAAAACTCGTTCGTTCATTT	39.1	30.0	58.2	52.2	1621-1641 bp	1.0	41.4
rpl16_p4	TCCCAAAACCTATTTCCTTTGGT	AAAACTCGTTCGTTCATTTT	39.1	30.0	58.2	52.2	1620-1640 bp	1.0	41.4
rpl16_p5	TCCCAAAACCTATTTCCTTTGGT	AAACTCGTTCGTTCATTTTT	39.1	30.0	58.2	52.2	1619-1639 bp	1.0	41.4