Hotspot

rbcL

LSC · 56117-57544 · 1428 bp

Back to Adinandra

Pi 0.00327

MarkerSeek score 56.30

Primer available yes

Label rbcL

Feature Metadata

label_name: rbcL
feature_type: gene
region: LSC
length_bp: 1428
variable_sites: 7
pi: 0.00327
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 56.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rbcL_p1	TCGAGTAGACCTTGTTGTTGT	TGGTAAAAGATTGGGCCGAG	42.9	50.0	56.9	57.9	1548 bp	1.0	62.7
rbcL_p2	TCGAGTAGACCTTGTTGTTGT	GGTAAAAGATTGGGCCGAGT	42.9	50.0	56.9	57.9	1547 bp	1.0	62.7
rbcL_p3	CGAGTAGACCTTGTTGTTGTG	TGGTAAAAGATTGGGCCGAG	47.6	50.0	56.9	57.9	1547 bp	1.0	62.3
rbcL_p4	CGAGTAGACCTTGTTGTTGTG	GGTAAAAGATTGGGCCGAGT	47.6	50.0	56.9	57.9	1546 bp	1.0	62.3
rbcL_p5	TCGAGTAGACCTTGTTGTTGT	TTCGGCTCAATCCTTTTGGT	42.9	45.0	56.9	57.7	1564 bp	1.0	61.4