Hotspot

petB

LSC · 76377-77799 · 1423 bp

Back to Adinandra

Pi 0.00234

MarkerSeek score 60.20

Primer available yes

Label petB

Feature Metadata

label_name: petB
feature_type: gene
region: LSC
length_bp: 1423
variable_sites: 5
pi: 0.00234
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petB_p1	TAGTTCGACCGCGGAATTTC	TGTTCCTCCCCCAAGTGATA	50.0	50.0	58.4	57.6	1525 bp	1.0	74.5
petB_p2	TTCGACCGCGGAATTTCTTT	TGTTCCTCCCCCAAGTGATA	45.0	50.0	58.5	57.6	1522 bp	1.0	73.6
petB_p3	TAGTTCGACCGCGGAATTTC	ATTGTTCCTCCCCCAAGTGA	50.0	50.0	58.4	58.5	1527 bp	1.0	73.0
petB_p4	TAGTTCGACCGCGGAATTTC	TTGTTCCTCCCCCAAGTGAT	50.0	50.0	58.4	58.5	1526 bp	1.0	73.0
petB_p5	AGCTCGGATTTCTAGCCCAT	TGTTCCTCCCCCAAGTGATA	50.0	50.0	58.6	57.6	1555 bp	1.0	72.9