Hotspot

ndhD

SSC · 117433-118932 · 1500 bp

Back to Adinandra

Pi 0.00267

MarkerSeek score 47.90

Primer available yes

Label ndhD

Feature Metadata

label_name: ndhD
feature_type: gene
region: SSC
length_bp: 1500
variable_sites: 6
pi: 0.00267
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 47.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhD_p1	TTGGACCGTATTCGCGATTT	AACCCGTGCTCGAGAATAAC	45.0	50.0	58.0	58.0	1999-2007 bp	1.0	80.6
ndhD_p2	TTGGACCGTATTCGCGATTT	TCTGCTCCAAGAACAGAGGA	45.0	50.0	58.0	58.0	2176-2184 bp	1.0	80.5
ndhD_p3	TTGGACCGTATTCGCGATTT	GGGACGGGTGTAAAGCTAAG	45.0	55.0	58.0	58.0	2205-2213 bp	1.0	80.5
ndhD_p4	TTGGACCGTATTCGCGATTT	ATACCTTGGGACGGGTGTAA	45.0	50.0	58.0	58.0	2212-2220 bp	1.0	80.3
ndhD_p5	TTACTTGGACCGTATTCGCG	AACCCGTGCTCGAGAATAAC	50.0	50.0	58.1	58.0	2003-2011 bp	1.0	80.0