Hotspot

matK

LSC · 2041-3567 · 1527 bp

Back to Adinandra

Pi 0.00131
MarkerSeek score 59.50
Primer available yes
Label matK

Feature Metadata

label_name
matK
feature_type
gene
region
LSC
length_bp
1527
variable_sites
3
pi
0.00131
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
59.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
matK_p1 ACATGCTACATGAACATTTCAGA CCTTGTTTTGACTGTATCGCA 34.8 42.9 56.5 56.8 1712 bp 1.0 40.1
matK_p2 ACATGCTACATGAACATTTCAGA ACCTTGTTTTGACTGTATCGC 34.8 42.9 56.5 56.8 1713 bp 1.0 40.1
matK_p3 ACATGCTACATGAACATTTCAGA CCTTGTTTTGACTGTATCGCAC 34.8 45.5 56.5 58.2 1712 bp 1.0 40.1
matK_p4 ACATGCTACATGAACATTTCAGAA CCTTGTTTTGACTGTATCGCA 33.3 42.9 57.2 56.8 1712 bp 1.0 40.1
matK_p5 ACATGCTACATGAACATTTCAGAA ACCTTGTTTTGACTGTATCGC 33.3 42.9 57.2 56.8 1713 bp 1.0 40.1