Hotspot

clpP

LSC · 71393-73455 · 2063 bp

Back to Adinandra

Pi 0.00194

MarkerSeek score 59.30

Primer available yes

Label clpP

Feature Metadata

label_name: clpP
feature_type: gene
region: LSC
length_bp: 2063
variable_sites: 6
pi: 0.00194
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
clpP_p1	GGGCTGGTTTAGATCGATCC	ACCCATTGTTACGTTTCCACA	55.0	42.9	57.8	58.1	2236-2245 bp	1.0	70.6
clpP_p2	GCGCGAAATCCATGCTATTT	ACCCATTGTTACGTTTCCACA	45.0	42.9	57.6	58.1	2137-2146 bp	1.0	68.8
clpP_p3	TGCGCGAAATCCATGCTATT	ACCCATTGTTACGTTTCCACA	45.0	42.9	58.7	58.1	2138-2147 bp	1.0	66.5
clpP_p4	TTGCGCGAAATCCATGCTAT	ACCCATTGTTACGTTTCCACA	45.0	42.9	58.7	58.1	2139-2148 bp	1.0	66.5
clpP_p5	GGGCTGGTTTAGATCGATCC	CCCATTGTTACGTTTCCACA	55.0	45.0	57.8	56.3	2235-2244 bp	1.0	65.2