Hotspot

ndhF-rpl32

SSC · 114020-114974 · 955 bp

Back to Adinandra

Pi 0.00558
MarkerSeek score 62.30
Primer available yes
Label ndhF-rpl32

Feature Metadata

label_name
ndhF-rpl32
feature_type
igs
region
SSC
length_bp
955
variable_sites
8
pi
0.00558
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
62.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhF-rpl32_p1 ACAAAAGGTATGATCCACGCA AGCTTTTAAGGCTGCCCAAT 42.9 45.0 57.9 58.1 1089-1093 bp 1.0 71.6
ndhF-rpl32_p2 ACAAAAGGTATGATCCACGCA AAAGCTTTTAAGGCTGCCCA 42.9 45.0 57.9 58.3 1091-1095 bp 1.0 69.8
ndhF-rpl32_p3 ACAAAAGGTATGATCCACGCA AAGCTTTTAAGGCTGCCCAA 42.9 45.0 57.9 58.3 1090-1094 bp 1.0 69.8
ndhF-rpl32_p4 ACAAAAGGTATGATCCACGCA AGGCTGCCCAATATCCTTTC 42.9 50.0 57.9 57.6 1081-1085 bp 1.0 69.1
ndhF-rpl32_p5 ACAAAAGGTATGATCCACGCA GCTGCCCAATATCCTTTCCT 42.9 50.0 57.9 57.6 1079-1083 bp 1.0 69.1