Hotspot

psaJ-rpl33

LSC · 68621-69075 · 455 bp

Back to Adinandra

Pi 0.00587

MarkerSeek score 49.40

Primer available yes

Label psaJ-rpl33

Feature Metadata

label_name: psaJ-rpl33
feature_type: igs
region: LSC
length_bp: 455
variable_sites: 4
pi: 0.00587
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 49.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaJ-rpl33_p1	ATGCGTTGACATTCCCCTTT	GGACATCTTTACCCTTGGCC	45.0	55.0	58.1	58.2	508-509 bp	1.0	78.3
psaJ-rpl33_p2	TGTGGCACCGGTACTAAGTA	GGACATCTTTACCCTTGGCC	50.0	55.0	58.1	58.2	585-586 bp	1.0	78.2
psaJ-rpl33_p3	CATATCTCTCTGTGGCACCG	GGACATCTTTACCCTTGGCC	55.0	55.0	57.9	58.2	595-596 bp	1.0	77.6
psaJ-rpl33_p4	TGGCACCGGTACTAAGTACT	GGACATCTTTACCCTTGGCC	50.0	55.0	57.8	58.2	583-584 bp	1.0	77.0
psaJ-rpl33_p5	GTTCGGGTCTTTAGCAGGTC	GGACATCTTTACCCTTGGCC	55.0	55.0	58.3	58.2	558-559 bp	1.0	76.6