Hotspot

psbC-trnS-UGA

LSC · 36109-36367 · 259 bp

Back to Adinandra

Pi 0.00563

MarkerSeek score 59.40

Primer available yes

Label psbC-trnS-UGA

Feature Metadata

label_name: psbC-trnS-UGA
feature_type: igs
region: LSC
length_bp: 259
variable_sites: 2
pi: 0.00563
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.92
markerseek_score: 59.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbC-trnS-UGA_p1	CCTGTTCTTTCCATGACCCC	GAGAGATGGCTGAGTGGTTG	55.0	55.0	58.2	58.0	359-381 bp	1.0	83.0
psbC-trnS-UGA_p2	CCTGTTCTTTCCATGACCCC	ATAGCCCCGGTCTTGAAAAC	55.0	50.0	58.2	57.9	339-361 bp	1.0	82.2
psbC-trnS-UGA_p3	CCTGTTCTTTCCATGACCCC	TCTTTCGGAGAGATGGCTGA	55.0	50.0	58.2	58.1	366-388 bp	1.0	82.0
psbC-trnS-UGA_p4	CCTGTTCTTTCCATGACCCC	GGGTTCGAATCCCTCTCTCT	55.0	55.0	58.2	58.2	290-312 bp	1.0	81.5
psbC-trnS-UGA_p5	CCTGTTCTTTCCATGACCCC	CTTTCGGAGAGATGGCTGAG	55.0	55.0	58.2	57.8	365-387 bp	1.0	81.4