Hotspot

petN-psbM

LSC · 29173-30278 · 1106 bp

Back to Adinandra

Pi 0.00663

MarkerSeek score 55.20

Primer available yes

Label petN-psbM

Feature Metadata

label_name: petN-psbM
feature_type: igs
region: LSC
length_bp: 1106
variable_sites: 11
pi: 0.00663
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 55.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petN-psbM_p1	TAGTAAGTCTCGCTTGGGCT	TGCTACTGCACTGTTCATTCT	50.0	42.9	58.2	57.6	1268-1284 bp	1.0	68.2
petN-psbM_p2	ATGGGGAAGAAGTGGACTCT	TGCTACTGCACTGTTCATTCT	50.0	42.9	57.6	57.6	1207-1223 bp	1.0	66.7
petN-psbM_p3	TAGTAAGTCTCGCTTGGGCT	TGCTACTGCACTGTTCATTC	50.0	45.0	58.2	56.1	1268-1284 bp	1.0	64.0
petN-psbM_p4	ATGGGGAAGAAGTGGACTCT	TGCTACTGCACTGTTCATTC	50.0	45.0	57.6	56.1	1207-1223 bp	1.0	62.5
petN-psbM_p5	ATAGTAAGTCTCGCTTGGGC	TGCTACTGCACTGTTCATTCT	50.0	42.9	57.1	57.6	1269-1285 bp	1.0	62.3