Hotspot

rps2-rpoC1

LSC · 16793-21349 · 4557 bp

Back to Adinandra

Pi 0.0022
MarkerSeek score 54.90
Primer available yes
Label rps2-rpoC1

Feature Metadata

label_name
rps2-rpoC1
feature_type
igs
region
LSC
length_bp
4557
variable_sites
15
pi
0.0022
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
54.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps2-rpoC1_p1 CCCCATAATGGCCATGAACA CCCGATCCATGCAGTAACAT 50.0 50.0 57.9 57.7 253-254 bp 1.0 78.8
rps2-rpoC1_p2 CATGAACAGTTGCTCCTGGA CCCGATCCATGCAGTAACAT 50.0 50.0 57.8 57.7 241-242 bp 1.0 78.1
rps2-rpoC1_p3 AATGGCCATGAACAGTTGCT CCCGATCCATGCAGTAACAT 45.0 50.0 58.4 57.7 247-248 bp 1.0 76.6
rps2-rpoC1_p4 CCCCATAATGGCCATGAACA ATCCCGATCCATGCAGTAAC 50.0 50.0 57.9 57.4 255-256 bp 1.0 76.5
rps2-rpoC1_p5 TCCCCATAATGGCCATGAAC CCCGATCCATGCAGTAACAT 50.0 50.0 57.6 57.7 254-255 bp 1.0 76.4