Hotspot

rps16-trnQ-UUG

LSC · 6313-7323 · 1011 bp

Back to Adinandra

Pi 0.00529
MarkerSeek score 59.30
Primer available yes
Label rps16-trnQ-UUG

Feature Metadata

label_name
rps16-trnQ-UUG
feature_type
igs
region
LSC
length_bp
1011
variable_sites
8
pi
0.00529
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
59.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps16-trnQ-UUG_p1 AACGGATCGTGTCCTTCAAG GAAATTGAAATGGGGCGTGG 50.0 50.0 57.9 58.0 1159-1200 bp 1.0 80.0
rps16-trnQ-UUG_p2 AAGTCGCACGTTGCTTTCTA GAAATTGAAATGGGGCGTGG 45.0 50.0 58.1 58.0 1142-1183 bp 1.0 79.6
rps16-trnQ-UUG_p3 AACGGATCGTGTCCTTCAAG GAGGTTCGAATCCTTCCGTC 50.0 55.0 57.9 58.1 1101-1142 bp 1.0 79.5
rps16-trnQ-UUG_p4 GTCGAGTCAAGAGCACCTTT GAAATTGAAATGGGGCGTGG 50.0 50.0 57.8 58.0 1213-1254 bp 1.0 79.4
rps16-trnQ-UUG_p5 AAGTCGCACGTTGCTTTCTA GAGGTTCGAATCCTTCCGTC 45.0 55.0 58.1 58.1 1084-1125 bp 1.0 79.1