Hotspot

trnH-GUG-psbA

LSC · 76-448 · 373 bp

Back to Adinandra

Pi 0.00542

MarkerSeek score 62.10

Primer available yes

Label trnH-GUG-psbA

Feature Metadata

label_name: trnH-GUG-psbA
feature_type: igs
region: LSC
length_bp: 373
variable_sites: 3
pi: 0.00542
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 62.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnH-GUG-psbA_p1	CTGCCTTAATCCACTTGGCT	CCTCTGGACCTAGCTGCTAT	50.0	55.0	57.9	58.0	435-446 bp	1.0	82.3
trnH-GUG-psbA_p2	ACTGCCTTAATCCACTTGGC	CCTCTGGACCTAGCTGCTAT	50.0	55.0	58.2	58.0	436-447 bp	1.0	82.1
trnH-GUG-psbA_p3	AATCCACTTGGCTACATCCG	CCTCTGGACCTAGCTGCTAT	50.0	55.0	57.7	58.0	428-439 bp	1.0	80.8
trnH-GUG-psbA_p4	CGCATGGTGGATTCACAATC	CCTCTGGACCTAGCTGCTAT	50.0	55.0	57.5	58.0	457-468 bp	1.0	79.4
trnH-GUG-psbA_p5	CTGCCTTAATCCACTTGGCT	CCGTGCTAACCTTGGTATGG	50.0	55.0	57.9	58.3	487-498 bp	1.0	79.3