Hotspot

rpl2

LSC · 79966-81530 · 1565 bp

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Pi 0.182

MarkerSeek score 84.20

Primer available yes

Label rpl2

Feature Metadata

label_name: rpl2
feature_type: gene
region: LSC
length_bp: 1565
variable_sites: 544
pi: 0.182
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 84.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl2_p1	TTTCGGGTGGGTACGAATTC	TGGCGAATTCACAGGTTCAA	50.0	45.0	57.9	58.0	2443-2570 bp	0.8	83.0
rpl2_p2	TTTCGGGTGGGTACGAATTC	TTGGCGAATTCACAGGTTCA	50.0	45.0	57.9	58.0	2444-2571 bp	0.8	83.0
rpl2_p3	TTTCGGGTGGGTACGAATTC	AGAAGTACGAAGTTGGGGGA	50.0	50.0	57.9	58.0	1895 bp	0.2	47.1
rpl2_p4	AATGCTCTCGGCCATTATGG	AGAAGTACGAAGTTGGGGGA	50.0	50.0	58.1	58.0	1831 bp	0.2	47.1
rpl2_p5	AATGCTCTCGGCCATTATGG	TGGCGAATTCACAGGTTCAA	50.0	45.0	58.1	58.0	2436 bp	0.2	47.0