Hotspot

petB

LSC · 70468-71820 · 1353 bp

Back to Adiantum

Pi 0.145

MarkerSeek score 84.00

Primer available yes

Label petB

Feature Metadata

label_name: petB
feature_type: gene
region: LSC
length_bp: 1353
variable_sites: 374
pi: 0.145
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 84.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petB_p1	GGCGTAACCGGTAAAGCTAA	ATCGTTTCTTTACCCCGACG	50.0	50.0	58.0	58.0	2494-2498 bp	0.4	66.2
petB_p2	TAAGGCGTAACCGGTAAAGC	ATCGTTTCTTTACCCCGACG	50.0	50.0	58.0	58.0	2501 bp	0.2	47.9
petB_p3	GGCGTAACCGGTAAAGCTAA	CCAGGTCCGGATGTAGAGAT	50.0	55.0	58.0	58.0	2389 bp	0.2	47.9
petB_p4	TAAGGCGTAACCGGTAAAGC	CCAGGTCCGGATGTAGAGAT	50.0	55.0	58.0	58.0	2392 bp	0.2	47.9
petB_p5	GGCGTAACCGGTAAAGCTAA	GTTGCGGAAAATTGGTAGCC	50.0	50.0	58.0	58.0	2469 bp	0.2	47.9