Hotspot

rps7-rps12

IRa · 137426-138048 · 623 bp

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Pi 0.0551

MarkerSeek score 83.40

Primer available yes

Label rps7-rps12

Feature Metadata

label_name: rps7-rps12
feature_type: igs
region: IRa
length_bp: 623
variable_sites: 70
pi: 0.0551
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 83.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps7-rps12_p1	AAGTAGTCGTCAACCCCTCA	AGCATTGTCGCAACTCGTAT	50.0	45.0	58.0	58.0	1243-1249 bp	0.6	69.9
rps7-rps12_p2	TCAACCCCTCACTCATCCAT	AGCATTGTCGCAACTCGTAT	50.0	45.0	58.0	58.0	1234-1244 bp	0.4	60.3
rps7-rps12_p3	GTAATGCACGCCCCTAATCA	AGCATTGTCGCAACTCGTAT	50.0	45.0	58.0	58.0	1390-1404 bp	0.4	58.9
rps7-rps12_p4	CTTCTTCCGTATGGCACTCC	AGCATTGTCGCAACTCGTAT	55.0	45.0	58.1	58.0	1064-1070 bp	0.4	58.0
rps7-rps12_p5	AAGTAGTCGTCAACCCCTCA	GTATCGGTAAACCCACGCAT	50.0	50.0	58.0	58.1	1768 bp	0.2	47.5