Hotspot

rpl32-trnP

SSC · 108939-109599 · 661 bp

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Pi 0.325

MarkerSeek score 82.50

Primer available yes

Label rpl32-trnP

Feature Metadata

label_name: rpl32-trnP
feature_type: igs
region: SSC
length_bp: 661
variable_sites: 380
pi: 0.325
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.94
markerseek_score: 82.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-trnP_p1	CAACTACGAGTACAACCGGG	AGAGCAGCGTGTCTACCTAT	55.0	50.0	58.0	57.9	1681-1716 bp	0.6	75.4
rpl32-trnP_p2	CAACTACGAGTACAACCGGG	TGGTTTGGAGGTAGCGAGTA	55.0	50.0	58.0	58.1	2065-2077 bp	0.4	65.6
rpl32-trnP_p3	CAACTACGAGTACAACCGGG	GGATTCGAACCGAGATGCTT	55.0	50.0	58.0	58.1	1716 bp	0.2	47.4
rpl32-trnP_p4	CAACTACGAGTACAACCGGG	CTATTTCACGTGCCGCTACT	55.0	50.0	58.0	58.1	1737 bp	0.2	47.3
rpl32-trnP_p5	CAACTACGAGTACAACCGGG	TATTTCACGTGCCGCTACTC	55.0	50.0	58.0	58.1	1736 bp	0.2	47.2