Hotspot

atpH-atpI

LSC · 13193-14001 · 809 bp

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Pi 0.246

MarkerSeek score 82.70

Primer available yes

Label atpH-atpI

Feature Metadata

label_name: atpH-atpI
feature_type: igs
region: LSC
length_bp: 809
variable_sites: 362
pi: 0.246
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 82.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpH-atpI_p1	CCCTCCTCGAAGGTTTTACG	GTTGGTAGTAGCTGTTCCCG	55.0	55.0	58.0	58.0	1531 bp	0.2	47.9
atpH-atpI_p2	ATAACTCGAGGGAGCGATGA	GTTGGTAGTAGCTGTTCCCG	50.0	55.0	58.0	58.0	1578 bp	0.2	47.8
atpH-atpI_p3	TAACTCGAGGGAGCGATGAT	GTTGGTAGTAGCTGTTCCCG	50.0	55.0	58.0	58.0	1577 bp	0.2	47.8
atpH-atpI_p4	CCCTCCTCGAAGGTTTTACG	ATTTTGGCTGACGAGTTGGT	55.0	45.0	58.0	58.0	1545 bp	0.2	47.8
atpH-atpI_p5	CCCTCCTCGAAGGTTTTACG	TCTTTGCCACTTTAGCTGCA	55.0	45.0	58.0	58.0	1436 bp	0.2	47.8