Hotspot

ycf1

IRb · 107352-107978 · 627 bp

Back to Adenostyles

Pi 0.00159

MarkerSeek score 59.70

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: IRb
length_bp: 627
variable_sites: 1
pi: 0.00159
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	AGAAAAGAAAAAGGGAGGTTTGTG	GCGGTGGTCGTATTTCTTCT	37.5	50.0	58.0	58.0	710 bp	1.0	47.9
ycf1_p2	AAGAAAAAGGGAGGTTTGTG	GCGGTGGTCGTATTTCTTCT	40.0	50.0	53.9	58.0	706 bp	1.0	47.1
ycf1_p3	AAAGAAAAAGGGAGGTTTGTG	GCGGTGGTCGTATTTCTTCT	38.1	50.0	54.8	58.0	707 bp	1.0	46.1
ycf1_p4	AGAAAAGAAAAAGGGAGGTTTGT	GCGGTGGTCGTATTTCTTCT	34.8	50.0	56.7	58.0	710 bp	1.0	45.8
ycf1_p5	GAAAAGAAAAAGGGAGGTTTGTG	GCGGTGGTCGTATTTCTTCT	39.1	50.0	56.7	58.0	709 bp	1.0	45.6