Hotspot

rpl14

LSC · 79716-80084 · 369 bp

Back to Adenostyles

Pi 0

MarkerSeek score 20.90

Primer available yes

Label rpl14

Feature Metadata

label_name: rpl14
feature_type: gene
region: LSC
length_bp: 369
variable_sites: 0
pi: 0
unique_haplotype_count: 1
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 20.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl14_p1	ACTCGAACCACGCTTTTTCT	GGTTACAGTAAGACCCGCAG	45.0	55.0	58.1	58.0	951 bp	1.0	79.8
rpl14_p2	ACTCGAACCACGCTTTTTCT	ATACGCTCTTCAGGCACTTG	45.0	50.0	58.1	58.0	1077 bp	1.0	79.7
rpl14_p3	ACTCGAACCACGCTTTTTCT	CGTCGCGGTGGAAAAATATG	45.0	50.0	58.1	57.9	995 bp	1.0	79.2
rpl14_p4	ACTCGAACCACGCTTTTTCT	CTTAAAAATCGCGGGTGCAG	45.0	50.0	58.1	58.1	748 bp	1.0	79.1
rpl14_p5	AGCCTCTCGGTCTGTCATTA	GGTTACAGTAAGACCCGCAG	50.0	55.0	57.9	58.0	1234 bp	1.0	79.0