Hotspot

ndhF

SSC · 107952-110177 · 2226 bp

Back to Adenostyles

Pi 0.0018

MarkerSeek score 58.30

Primer available yes

Label ndhF

Feature Metadata

label_name: ndhF
feature_type: gene
region: SSC
length_bp: 2226
variable_sites: 4
pi: 0.0018
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 58.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF_p1	TTCGATGCAACAGCAAGATG	TCGAAAGAGGTAAAATCCGGT	45.0	42.9	57.1	57.0	2405 bp	1.0	57.1
ndhF_p2	TTCGATGCAACAGCAAGATG	CGAAAGAGGTAAAATCCGGTG	45.0	47.6	57.1	57.0	2404 bp	1.0	56.8
ndhF_p3	CGATGCAACAGCAAGATGC	TCGAAAGAGGTAAAATCCGGT	52.6	42.9	58.1	57.0	2403 bp	1.0	55.9
ndhF_p4	CGATGCAACAGCAAGATGC	CGAAAGAGGTAAAATCCGGTG	52.6	47.6	58.1	57.0	2402 bp	1.0	55.6
ndhF_p5	TTCGATGCAACAGCAAGATG	AGAGGTAAAATCCGGTGAATCG	45.0	45.5	57.1	58.2	2400 bp	1.0	55.2