Hotspot

cemA

LSC · 60332-61021 · 690 bp

Back to Adenostyles

Pi 0.00145

MarkerSeek score 59.60

Primer available yes

Label cemA

Feature Metadata

label_name: cemA
feature_type: gene
region: LSC
length_bp: 690
variable_sites: 1
pi: 0.00145
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
cemA_p1	GAGTTGACGGATGAGATGGG	CGCGGGATGGCTAGAAATAT	55.0	50.0	57.8	57.6	821 bp	1.0	75.2
cemA_p2	GAGTTGACGGATGAGATGGG	AGCAATTTTCACCGCTTTGC	55.0	45.0	57.8	58.5	799 bp	1.0	74.3
cemA_p3	GAGTTGACGGATGAGATGGG	GCAATTTTCACCGCTTTGCT	55.0	45.0	57.8	58.5	798 bp	1.0	74.3
cemA_p4	GAGTTGACGGATGAGATGGG	ATATAGGAGGAATCCGCGGG	55.0	55.0	57.8	58.5	835 bp	1.0	74.1
cemA_p5	GTTGACGGATGAGATGGGTT	CGCGGGATGGCTAGAAATAT	50.0	50.0	57.6	57.6	819 bp	1.0	73.8