Hotspot

atpF

LSC · 27012-28269 · 1258 bp

Back to Adenostyles

Pi 0.00239

MarkerSeek score 59.10

Primer available yes

Label atpF

Feature Metadata

label_name: atpF
feature_type: gene
region: LSC
length_bp: 1258
variable_sites: 3
pi: 0.00239
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpF_p1	GGGCAAAGTAAAAAGAACTCTGT	ATTTCGTCGGCTTGAATGGT	39.1	45.0	57.5	58.2	1413-1415 bp	1.0	50.7
atpF_p2	GGGCAAAGTAAAAAGAACTCTGT	GTCGGCTTGAATGGTTACCA	39.1	50.0	57.5	58.2	1408-1410 bp	1.0	50.7
atpF_p3	GGGCAAAGTAAAAAGAACTCTGT	TCGTCGGCTTGAATGGTTAC	39.1	50.0	57.5	58.3	1410-1412 bp	1.0	49.9
atpF_p4	GGGCAAAGTAAAAAGAACTCTGT	TTTCGTCGGCTTGAATGGTT	39.1	45.0	57.5	58.4	1412-1414 bp	1.0	49.0
atpF_p5	AGGGCAAAGTAAAAAGAACTCTG	ATTTCGTCGGCTTGAATGGT	39.1	45.0	57.2	58.2	1414-1416 bp	1.0	48.7