Hotspot

ndhC-trnV-UAC

LSC · 50313-51069 · 757 bp

Back to Adenostyles

Pi 0.00396
MarkerSeek score 61.00
Primer available yes
Label ndhC-trnV-UAC

Feature Metadata

label_name
ndhC-trnV-UAC
feature_type
igs
region
LSC
length_bp
757
variable_sites
3
pi
0.00396
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
61.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhC-trnV-UAC_p1 ACTCGGAGCTAAAAATCCCG AGAAGGTCTACGGTTCGAGT 50.0 50.0 57.7 57.8 892 bp 1.0 76.2
ndhC-trnV-UAC_p2 CTCGGAGCTAAAAATCCCGA AGAAGGTCTACGGTTCGAGT 50.0 50.0 57.4 57.8 891 bp 1.0 73.9
ndhC-trnV-UAC_p3 ACTCGGAGCTAAAAATCCCG GAAGGTCTACGGTTCGAGTC 50.0 55.0 57.7 57.5 891 bp 1.0 73.5
ndhC-trnV-UAC_p4 ACTCGGAGCTAAAAATCCCG GTTCGAGTCCGTATAGCCCT 50.0 55.0 57.7 58.7 880 bp 1.0 72.3
ndhC-trnV-UAC_p5 CCCTTTGCTACTCGGAGCTA AGAAGGTCTACGGTTCGAGT 55.0 50.0 58.9 57.8 901 bp 1.0 71.6