Hotspot

psbM-trnD-GUC

LSC · 11038-11650 · 613 bp

Back to Adenostyles

Pi 0.00164
MarkerSeek score 60.00
Primer available yes
Label psbM-trnD-GUC

Feature Metadata

label_name
psbM-trnD-GUC
feature_type
igs
region
LSC
length_bp
613
variable_sites
1
pi
0.00164
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
60.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbM-trnD-GUC_p1 GGAACTAAAATGAACAGTGCAGT TTCAATAGGCTAGAGCACCG 39.1 50.0 57.6 57.1 731-732 bp 1.0 45.7
psbM-trnD-GUC_p2 AAAATGAACAGTGCAGTAGC TTCAATAGGCTAGAGCACCG 40.0 50.0 54.5 57.1 725-726 bp 1.0 45.2
psbM-trnD-GUC_p3 ACTAAAATGAACAGTGCAGTAGC TTCAATAGGCTAGAGCACCG 39.1 50.0 57.4 57.1 728-729 bp 1.0 44.3
psbM-trnD-GUC_p4 GGAACTAAAATGAACAGTGCAGT CAATAGGCTAGAGCACCGC 39.1 57.9 57.6 58.1 729-730 bp 1.0 44.1
psbM-trnD-GUC_p5 AGGAACTAAAATGAACAGTGCAG TTCAATAGGCTAGAGCACCG 39.1 50.0 57.3 57.1 732-733 bp 1.0 43.6