Hotspot

rps16-trnQ-UUG

LSC · 6373-7341 · 969 bp

Back to Adenostyles

Pi 0.00311

MarkerSeek score 61.00

Primer available yes

Label rps16-trnQ-UUG

Feature Metadata

label_name: rps16-trnQ-UUG
feature_type: igs
region: LSC
length_bp: 969
variable_sites: 3
pi: 0.00311
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 61.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps16-trnQ-UUG_p1	AAGTCGCACGTTGCTTTCTA	GAGGTTCGAATCCTTCCGTC	45.0	55.0	58.1	58.1	1037-1042 bp	1.0	78.9
rps16-trnQ-UUG_p2	AAGTCGCACGTTGCTTTCTA	GGTAAGGCATCGGGTTTTGA	45.0	50.0	58.1	58.2	1069-1074 bp	1.0	78.1
rps16-trnQ-UUG_p3	AGAATAAGAATCCACGCCGG	GAGGTTCGAATCCTTCCGTC	50.0	55.0	57.8	58.1	1069-1074 bp	1.0	78.1
rps16-trnQ-UUG_p4	AGAATAAGAATCCACGCCGG	GGTAAGGCATCGGGTTTTGA	50.0	50.0	57.8	58.2	1101-1106 bp	1.0	77.3
rps16-trnQ-UUG_p5	GGATCGTGTCCTTCAAGTCG	GAGGTTCGAATCCTTCCGTC	55.0	55.0	58.4	58.1	1051-1056 bp	1.0	77.0