Hotspot

ycf1

SSC · 106405-111447 · 5043 bp

Back to Adenostemma

Pi 0.381

MarkerSeek score 73.00

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5043
variable_sites: 1513
pi: 0.381
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.79
markerseek_score: 73.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	ATGGTCTCCTGGTTAGCCTC	AACATACTAAAGCGGCTCCC	55.0	50.0	58.5	57.7	607 bp	0.5	53.4
ycf1_p2	GGTCTCCTGGTTAGCCTCAT	AACATACTAAAGCGGCTCCC	55.0	50.0	58.5	57.7	605 bp	0.5	53.4
ycf1_p3	ATGGTCTCCTGGTTAGCCTC	GGCTCCCATTATTGGTGTCA	55.0	50.0	58.5	57.6	594 bp	0.5	52.5
ycf1_p4	GGTCTCCTGGTTAGCCTCAT	GGCTCCCATTATTGGTGTCA	55.0	50.0	58.5	57.6	592 bp	0.5	52.5
ycf1_p5	GGTCTCCTGGTTAGCCTCAT	CATACTAAAGCGGCTCCCAT	55.0	50.0	58.5	57.4	603 bp	0.5	51.6