Hotspot

ndhH

SSC · 112209-113390 · 1182 bp

Back to Adenostemma

Pi 0.474

MarkerSeek score 81.40

Primer available yes

Label ndhH

Feature Metadata

label_name: ndhH
feature_type: gene
region: SSC
length_bp: 1182
variable_sites: 488
pi: 0.474
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.87
markerseek_score: 81.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhH_p1	CGTCAACGGTTACTGGCTTA	CGTTGTTGTATACCCGCAGA	50.0	50.0	57.9	58.0	1549 bp	0.5	59.4
ndhH_p2	CGTCAACGGTTACTGGCTTA	AAGAATTCCCAAAGGCCCTG	50.0	50.0	57.9	58.1	1586 bp	0.5	59.0
ndhH_p3	CGTCAACGGTTACTGGCTTA	CCTGACATAAGAAGGCCGAC	50.0	55.0	57.9	58.1	1813 bp	0.5	59.0
ndhH_p4	CGTCAACGGTTACTGGCTTA	GACATAAGAAGGCCGACAGG	50.0	55.0	57.9	58.1	1810 bp	0.5	59.0
ndhH_p5	CGTCAACGGTTACTGGCTTA	TGAAGAATTCCCAAAGGCCC	50.0	50.0	57.9	58.1	1588 bp	0.5	58.9