Hotspot

ndhF

SSC · 122853-125078 · 2226 bp

Back to Adenostemma

Pi 0.452

MarkerSeek score 80.40

Primer available yes

Label ndhF

Feature Metadata

label_name: ndhF
feature_type: gene
region: SSC
length_bp: 2226
variable_sites: 895
pi: 0.452
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.89
markerseek_score: 80.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF_p1	AGGTAAGATCCGGTGAATCG	GCCGAGAAGAAGGTATCAGC	50.0	55.0	56.8	58.1	2708 bp	0.5	49.4
ndhF_p2	AGGTAAGATCCGGTGAATCG	CTAGCACTGGGTAGACCTCA	50.0	55.0	56.8	57.9	2624 bp	0.5	49.4
ndhF_p3	AGGTAAGATCCGGTGAATCG	CGCCTCTTCATCTAGCACTG	50.0	55.0	56.8	58.1	2635 bp	0.5	49.4
ndhF_p4	AGGTAAGATCCGGTGAATCG	TCGTTGTGGTCGGACTCTAT	50.0	50.0	56.8	58.2	2812 bp	0.5	49.2
ndhF_p5	AGGTAAGATCCGGTGAATCG	ATCAATTCGGTCGTTGTGGT	50.0	45.0	56.8	57.8	2822 bp	0.5	49.1