Hotspot

ndhD

SSC · 118130-119632 · 1503 bp

Back to Adenostemma

Pi 0.488

MarkerSeek score 82.00

Primer available yes

Label ndhD

Feature Metadata

label_name: ndhD
feature_type: gene
region: SSC
length_bp: 1503
variable_sites: 655
pi: 0.488
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.89
markerseek_score: 82.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhD_p1	TCTGCTCCAAGAACAGAGGA	ATTCACGCGGTTCTCGTAAA	50.0	45.0	58.0	57.9	1915 bp	0.5	59.3
ndhD_p2	TCTGCTCCAAGAACAGAGGA	TCAGGAGCAGTATGGGCTAA	50.0	50.0	58.0	57.8	2268 bp	0.5	58.5
ndhD_p3	TCTGCTCCAAGAACAGAGGA	TTCAGGAGCAGTATGGGCTA	50.0	50.0	58.0	57.8	2269 bp	0.5	58.5
ndhD_p4	TGATACCTTGGGACGGATGT	ATTCACGCGGTTCTCGTAAA	50.0	45.0	58.1	57.9	1953 bp	0.5	58.4
ndhD_p5	TCTGCTCCAAGAACAGAGGA	GCGGTTCTCGTAAAGACCAA	50.0	50.0	58.0	58.2	1909 bp	0.5	58.2