Hotspot

ndhA

SSC · 113392-115537 · 2146 bp

Back to Adenostemma

Pi 0.483

MarkerSeek score 78.70

Primer available yes

Label ndhA

Feature Metadata

label_name: ndhA
feature_type: gene
region: SSC
length_bp: 2146
variable_sites: 852
pi: 0.483
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.82
markerseek_score: 78.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhA_p1	GTGGAGTCGCTGCTGATTTA	TGCAGCTCGTACTGTTTGTT	50.0	45.0	58.0	58.1	2983 bp	0.5	59.5
ndhA_p2	GTGGAGTCGCTGCTGATTTA	TTGCAGCTCGTACTGTTTGT	50.0	45.0	58.0	58.1	2984 bp	0.5	59.5
ndhA_p3	TGGAGTCGCTGCTGATTTAC	TGCAGCTCGTACTGTTTGTT	50.0	45.0	58.0	58.1	2982 bp	0.5	59.5
ndhA_p4	TGGAGTCGCTGCTGATTTAC	TTGCAGCTCGTACTGTTTGT	50.0	45.0	58.0	58.1	2983 bp	0.5	59.5
ndhA_p5	TCAACAGGCCCTAGAAGGAA	TGCAGCTCGTACTGTTTGTT	50.0	45.0	58.0	58.1	2640 bp	0.5	59.3