Hotspot

trnL-UAG-rpl32

SSC · 121079-121819 · 741 bp

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Pi 0.443
MarkerSeek score 83.30
Primer available yes
Label trnL-UAG-rpl32

Feature Metadata

label_name
trnL-UAG-rpl32
feature_type
igs
region
SSC
length_bp
741
variable_sites
308
pi
0.443
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.94
markerseek_score
83.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnL-UAG-rpl32_p1 AGCGTGTCTACCGATTTCAC AAAGGAAAGGATATGGGGCG 50.0 50.0 58.0 57.6 874 bp 0.5 57.0
trnL-UAG-rpl32_p2 CAGCGTGTCTACCGATTTCA AAAGGAAAGGATATGGGGCG 50.0 50.0 58.0 57.6 875 bp 0.5 57.0
trnL-UAG-rpl32_p3 AGCGTGTCTACCGATTTCAC CGGCGTTAAAAGCTTTGTCA 50.0 45.0 58.0 57.6 856 bp 0.5 56.8
trnL-UAG-rpl32_p4 CAGCGTGTCTACCGATTTCA CGGCGTTAAAAGCTTTGTCA 50.0 45.0 58.0 57.6 857 bp 0.5 56.8
trnL-UAG-rpl32_p5 GAAGTAGCAAAGCCTTGGGT AAAGGAAAGGATATGGGGCG 50.0 50.0 58.1 57.6 1393 bp 0.5 56.4