Hotspot

ccsA-trnL-UAG

SSC · 120875-120998 · 124 bp

Back to Adenostemma

Pi 0.438

MarkerSeek score 74.80

Primer available yes

Label ccsA-trnL-UAG

Feature Metadata

label_name: ccsA-trnL-UAG
feature_type: igs
region: SSC
length_bp: 124
variable_sites: 53
pi: 0.438
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 74.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ccsA-trnL-UAG_p1	GAAGTAGCAAAGCCTTGGGT	GTGAAATCGGTAGACACGCT	50.0	50.0	58.1	58.0	539 bp	0.5	59.2
ccsA-trnL-UAG_p2	GAAGTAGCAAAGCCTTGGGT	TGAAATCGGTAGACACGCTG	50.0	50.0	58.1	58.0	538 bp	0.5	59.2
ccsA-trnL-UAG_p3	GAAGTAGCAAAGCCTTGGGT	AATTTTGGTGGTGGGGAGTC	50.0	50.0	58.1	58.0	1062 bp	0.5	59.2
ccsA-trnL-UAG_p4	GAAGTAGCAAAGCCTTGGGT	TGGTGGTGGGGAGTCTTATT	50.0	50.0	58.1	57.9	1057 bp	0.5	58.9
ccsA-trnL-UAG_p5	GAAGTAGCAAAGCCTTGGGT	TTGGTGGTGGGGAGTCTTAT	50.0	50.0	58.1	57.9	1058 bp	0.5	58.9