Hotspot

rps15-ndhH

SSC · 112121-112208 · 88 bp

Back to Adenostemma

Pi 0.447

MarkerSeek score 75.20

Primer available yes

Label rps15-ndhH

Feature Metadata

label_name: rps15-ndhH
feature_type: igs
region: SSC
length_bp: 88
variable_sites: 34
pi: 0.447
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.86
markerseek_score: 75.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps15-ndhH_p1	CGTCAACGGTTACTGGCTTA	GTAAATCAGCAGCGACTCCA	50.0	50.0	57.9	58.0	686 bp	0.5	59.4
rps15-ndhH_p2	CGTCAACGGTTACTGGCTTA	TAAATCAGCAGCGACTCCAC	50.0	50.0	57.9	58.0	685 bp	0.5	59.4
rps15-ndhH_p3	CGTCAACGGTTACTGGCTTA	AACACCATGCATTGATGGGT	50.0	45.0	57.9	58.1	259 bp	0.5	59.0
rps15-ndhH_p4	CGTCAACGGTTACTGGCTTA	AGGAGTCTGTGCCCCAATAT	50.0	50.0	57.9	58.1	574 bp	0.5	58.6
rps15-ndhH_p5	AGGTCTGCGGAAAATTCTGG	TAAATCAGCAGCGACTCCAC	50.0	50.0	58.2	58.0	710 bp	0.5	58.5