Hotspot

ycf1

SSC · 109252-114777 · 5526 bp

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Pi 0.353

MarkerSeek score 76.30

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5526
variable_sites: 1756
pi: 0.353
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.90
markerseek_score: 76.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	CTCGACCAAAATCCCCCAAA	ACATACTGAAACGACTGCCA	50.0	45.0	58.1	57.2	1110 bp	0.5	52.7
ycf1_p2	TCGACCAAAATCCCCCAAAG	ACATACTGAAACGACTGCCA	50.0	45.0	58.1	57.2	1109 bp	0.5	52.7
ycf1_p3	AAAGAACCCACAAGCCAAGT	ACATACTGAAACGACTGCCA	45.0	45.0	57.8	57.2	841 bp	0.5	52.1
ycf1_p4	GGGACGAGACTTTGAACCAA	ACATACTGAAACGACTGCCA	50.0	45.0	57.8	57.2	666 bp	0.5	51.3
ycf1_p5	GACCAAAATCCCCCAAAGGA	ACATACTGAAACGACTGCCA	50.0	45.0	57.7	57.2	1107 bp	0.5	50.8