Hotspot

ndhI

SSC · 118961-119458 · 498 bp

Back to Adenosma

Pi 0.489

MarkerSeek score 79.40

Primer available yes

Label ndhI

Feature Metadata

label_name: ndhI
feature_type: gene
region: SSC
length_bp: 498
variable_sites: 213
pi: 0.489
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.88
markerseek_score: 79.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhI_p1	GGAGCCGTATGAGGTGAAAG	AATCATGTATTGGTCCGGGC	55.0	50.0	58.1	58.0	1575 bp	0.5	59.4
ndhI_p2	GGAGCCGTATGAGGTGAAAG	ATAAGACCCGGTCCCAGAAA	55.0	50.0	58.1	58.0	1606 bp	0.5	59.2
ndhI_p3	GGAGCCGTATGAGGTGAAAG	CGCAGCAGCTACAAAATACG	55.0	50.0	58.1	57.9	1730 bp	0.5	58.9
ndhI_p4	GGAGCCGTATGAGGTGAAAG	ATGATCCCATACCAGGACGT	55.0	50.0	58.1	57.9	1918 bp	0.5	58.7
ndhI_p5	GGTTCTGTAATAGCGACGGG	AATCATGTATTGGTCCGGGC	55.0	50.0	58.2	58.0	1545 bp	0.5	58.6