Hotspot

ndhF

SSC · 126188-128455 · 2268 bp

Back to Adenosma

Pi 0.453

MarkerSeek score 78.10

Primer available yes

Label ndhF

Feature Metadata

label_name: ndhF
feature_type: gene
region: SSC
length_bp: 2268
variable_sites: 824
pi: 0.453
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.80
markerseek_score: 78.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF_p1	CACCCCTTGCAAAAGAGGTA	AGGGTGGAAAGTGAGGAAGA	50.0	50.0	57.7	57.9	2673 bp	0.5	56.8
ndhF_p2	TTCACCCCTTGCAAAAGAGG	AGGGTGGAAAGTGAGGAAGA	50.0	50.0	58.3	57.9	2675 bp	0.5	56.7
ndhF_p3	CACCCCTTGCAAAAGAGGTA	ACAGCTTCCGAAATGAAGGG	50.0	50.0	57.7	58.2	2634 bp	0.5	56.3
ndhF_p4	TTCACCCCTTGCAAAAGAGG	ACAGCTTCCGAAATGAAGGG	50.0	50.0	58.3	58.2	2636 bp	0.5	56.2
ndhF_p5	CACCCCTTGCAAAAGAGGTA	TAGGCAGAATACCCTCACCC	50.0	55.0	57.7	58.2	2735 bp	0.5	56.1