Hotspot

ndhE

SSC · 120578-120883 · 306 bp

Back to Adenosma

Pi 0.465

MarkerSeek score 77.30

Primer available yes

Label ndhE

Feature Metadata

label_name: ndhE
feature_type: gene
region: SSC
length_bp: 306
variable_sites: 134
pi: 0.465
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.94
markerseek_score: 77.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhE_p1	TTTCTGGGACCGGGTCTTAT	TACATCCGTCCCAGGGTATC	50.0	55.0	58.0	58.0	1393 bp	0.5	59.6
ndhE_p2	TTTCTGGGACCGGGTCTTAT	CCGAATTGGTTTAGGAGCGA	50.0	50.0	58.0	58.0	1177 bp	0.5	59.5
ndhE_p3	TTTCTGGGACCGGGTCTTAT	TCCGAATTGGTTTAGGAGCG	50.0	50.0	58.0	58.0	1178 bp	0.5	59.5
ndhE_p4	CGTATTTTGTAGCTGCTGCG	TACATCCGTCCCAGGGTATC	50.0	55.0	57.9	58.0	1269 bp	0.5	59.3
ndhE_p5	CGTATTTTGTAGCTGCTGCG	CCGAATTGGTTTAGGAGCGA	50.0	50.0	57.9	58.0	1053 bp	0.5	59.2