Hotspot

ccsA

SSC · 123241-124209 · 969 bp

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Pi 0.463

MarkerSeek score 78.20

Primer available yes

Label ccsA

Feature Metadata

label_name: ccsA
feature_type: gene
region: SSC
length_bp: 969
variable_sites: 340
pi: 0.463
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.76
markerseek_score: 78.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ccsA_p1	TGTCCGGGTTCTCACTAGTT	GCGGCATGTCATCTCCTAAA	50.0	50.0	58.0	58.0	1159 bp	0.5	59.6
ccsA_p2	TTGTCCGGGTTCTCACTAGT	GCGGCATGTCATCTCCTAAA	50.0	50.0	58.0	58.0	1160 bp	0.5	59.6
ccsA_p3	GTAGCTTTTCGATGGCCTCA	GCGGCATGTCATCTCCTAAA	50.0	50.0	58.0	58.0	1683 bp	0.5	59.4
ccsA_p4	TAGCTTTTCGATGGCCTCAC	GCGGCATGTCATCTCCTAAA	50.0	50.0	58.0	58.0	1682 bp	0.5	59.4
ccsA_p5	TGTCCGGGTTCTCACTAGTT	CTCGCCATCAATCGACTCAA	50.0	50.0	58.0	58.1	1696 bp	0.5	59.4