Hotspot

rpl32-ndhF

SSC · 125390-126187 · 798 bp

Back to Adenosma

Pi 0.438

MarkerSeek score 80.80

Primer available yes

Label rpl32-ndhF

Feature Metadata

label_name: rpl32-ndhF
feature_type: igs
region: SSC
length_bp: 798
variable_sites: 298
pi: 0.438
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.85
markerseek_score: 80.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-ndhF_p1	TTGAGTCGATTGATGGCGAG	CAAGAGGTCGTGTGAACCAA	50.0	50.0	58.1	58.1	1857 bp	0.5	59.0
rpl32-ndhF_p2	CCCACCTTGGAACTTGGAAA	CAAGAGGTCGTGTGAACCAA	50.0	50.0	57.9	58.1	1634 bp	0.5	58.9
rpl32-ndhF_p3	AAAACTCCCCACCTTGGAAC	CAAGAGGTCGTGTGAACCAA	50.0	50.0	57.9	58.1	1641 bp	0.5	58.9
rpl32-ndhF_p4	TTGAGTCGATTGATGGCGAG	GCCCACATACGACGAAGATT	50.0	50.0	58.1	58.1	1468 bp	0.5	58.9
rpl32-ndhF_p5	CCCACCTTGGAACTTGGAAA	GCCCACATACGACGAAGATT	50.0	50.0	57.9	58.1	1245 bp	0.5	58.8