Hotspot

trnL-UAG-rpl32

SSC · 124399-125197 · 799 bp

Back to Adenosma

Pi 0.455

MarkerSeek score 78.20

Primer available yes

Label trnL-UAG-rpl32

Feature Metadata

label_name: trnL-UAG-rpl32
feature_type: igs
region: SSC
length_bp: 799
variable_sites: 276
pi: 0.455
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.76
markerseek_score: 78.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnL-UAG-rpl32_p1	TTTAGGAGATGACATGCCGC	AAAAAGCGCACTTCTGCATC	50.0	45.0	58.0	57.9	1073 bp	0.5	59.2
trnL-UAG-rpl32_p2	TGATCCACATAAAAGGGCCG	AAAAAGCGCACTTCTGCATC	50.0	45.0	57.9	57.9	1630 bp	0.5	59.1
trnL-UAG-rpl32_p3	AACCACTCGGATTGAAGAGC	AAAAAGCGCACTTCTGCATC	50.0	45.0	57.9	57.9	1575 bp	0.5	58.7
trnL-UAG-rpl32_p4	TCCTAAGAGCAGCGTGTCTA	AAAAAGCGCACTTCTGCATC	50.0	45.0	57.9	57.9	1018 bp	0.5	58.6
trnL-UAG-rpl32_p5	AAAAGGGCCGCATAGCTTAA	AAAAAGCGCACTTCTGCATC	45.0	45.0	57.9	57.9	1620 bp	0.5	58.4