Hotspot

psaC-ndhD

SSC · 121386-121479 · 94 bp

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Pi 0.465

MarkerSeek score 76.40

Primer available yes

Label psaC-ndhD

Feature Metadata

label_name: psaC-ndhD
feature_type: igs
region: SSC
length_bp: 94
variable_sites: 40
pi: 0.465
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.91
markerseek_score: 76.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaC-ndhD_p1	GATACCCTGGGACGGATGTA	CCTATTTGCCCGCTGTACAT	55.0	50.0	58.0	58.0	664 bp	0.5	59.7
psaC-ndhD_p2	CGCTCCTAAACCAATTCGGA	CCTATTTGCCCGCTGTACAT	50.0	50.0	58.0	58.0	879 bp	0.5	59.6
psaC-ndhD_p3	TCGCTCCTAAACCAATTCGG	CCTATTTGCCCGCTGTACAT	50.0	50.0	58.0	58.0	880 bp	0.5	59.6
psaC-ndhD_p4	GATACCCTGGGACGGATGTA	ACCAATACCCAGAACTCCCA	55.0	50.0	58.0	57.9	842 bp	0.5	59.5
psaC-ndhD_p5	CGCTCCTAAACCAATTCGGA	ACCAATACCCAGAACTCCCA	50.0	50.0	58.0	57.9	1057 bp	0.5	59.4