Hotspot

ndhI-ndhG

SSC · 119459-119810 · 352 bp

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Pi 0.498

MarkerSeek score 76.60

Primer available yes

Label ndhI-ndhG

Feature Metadata

label_name: ndhI-ndhG
feature_type: igs
region: SSC
length_bp: 352
variable_sites: 155
pi: 0.498
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.88
markerseek_score: 76.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhI-ndhG_p1	CGAGGCCGAATACACTTTGA	AATCATGTATTGGTCCGGGC	50.0	50.0	58.0	58.0	728 bp	0.5	59.8
ndhI-ndhG_p2	CGAGGCCGAATACACTTTGA	ATAAGACCCGGTCCCAGAAA	50.0	50.0	58.0	58.0	759 bp	0.5	59.7
ndhI-ndhG_p3	ATGATAACCTTGTCCCACGC	AATCATGTATTGGTCCGGGC	50.0	50.0	58.0	58.0	809 bp	0.5	59.5
ndhI-ndhG_p4	ATGATAACCTTGTCCCACGC	ATAAGACCCGGTCCCAGAAA	50.0	50.0	58.0	58.0	840 bp	0.5	59.4
ndhI-ndhG_p5	CGAGGCCGAATACACTTTGA	CGCAGCAGCTACAAAATACG	50.0	50.0	58.0	57.9	883 bp	0.5	59.3