Hotspot

ycf1-rps15

SSC · 114778-115162 · 385 bp

Back to Adenosma

Pi 0.439

MarkerSeek score 75.90

Primer available yes

Label ycf1-rps15

Feature Metadata

label_name: ycf1-rps15
feature_type: igs
region: SSC
length_bp: 385
variable_sites: 141
pi: 0.439
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.83
markerseek_score: 75.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1-rps15_p1	GGGACGAGACTTTGAACCAA	TCCCAACGCGTTACATAAGG	50.0	50.0	57.8	58.0	1680 bp	0.5	57.9
ycf1-rps15_p2	GGGACGAGACTTTGAACCAA	AACACCATGCATTGATGGGT	50.0	45.0	57.8	58.1	1543 bp	0.5	57.5
ycf1-rps15_p3	GGGACGAGACTTTGAACCAA	TGCATTGATGGGTGATGAGG	50.0	50.0	57.8	57.9	1536 bp	0.5	57.5
ycf1-rps15_p4	GGGACGAGACTTTGAACCAA	GCATTGATGGGTGATGAGGT	50.0	50.0	57.8	57.9	1535 bp	0.5	57.5
ycf1-rps15_p5	GGGACGAGACTTTGAACCAA	CCCAGAATTTTCCGCAAACC	50.0	50.0	57.8	57.9	1271 bp	0.5	57.4