Hotspot

trnS-GCU-trnG-UCC

LSC · 8039-8854 · 816 bp

Back to Adenosma

Pi 0.0384

MarkerSeek score 78.30

Primer available yes

Label trnS-GCU-trnG-UCC

Feature Metadata

label_name: trnS-GCU-trnG-UCC
feature_type: igs
region: LSC
length_bp: 816
variable_sites: 30
pi: 0.0384
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 78.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-GCU-trnG-UCC_p1	CCGACGCTTTAATCCACTCA	ACCGAAAGACCCTTTAACTGT	50.0	42.9	58.0	57.1	899-917 bp	1.0	70.9
trnS-GCU-trnG-UCC_p2	ACGGAAAGAGAGGGATTCGA	ACCGAAAGACCCTTTAACTGT	50.0	42.9	58.2	57.1	957-975 bp	1.0	69.4
trnS-GCU-trnG-UCC_p3	ACGCTTTAATCCACTCAGCC	ACCGAAAGACCCTTTAACTGT	50.0	42.9	58.3	57.1	896-914 bp	1.0	68.8
trnS-GCU-trnG-UCC_p4	CGCTTTAATCCACTCAGCCA	ACCGAAAGACCCTTTAACTGT	50.0	42.9	58.3	57.1	895-913 bp	1.0	68.8
trnS-GCU-trnG-UCC_p5	TGGAAACGGAAAGAGAGGGA	ACCGAAAGACCCTTTAACTGT	50.0	42.9	58.3	57.1	962-980 bp	1.0	68.3