Hotspot

rps12-ycf3

Genome · 163043-163974 · 932 bp

Back to Adenophora

Pi 0.0502

MarkerSeek score 77.90

Primer available yes

Label rps12-ycf3

Feature Metadata

label_name: rps12-ycf3
feature_type: igs
region: Genome
length_bp: 932
variable_sites: 114
pi: 0.0502
unique_haplotype_count: 8
primer_available: yes
alignment_reliability: 0.89
markerseek_score: 77.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps12-ycf3_p1	TAAGAGCGGGGGATTTTGTG	GGTAAAGCGGAAGAAGCCTT	50.0	50.0	57.9	58.2	397-1930 bp	1.0	88.3
rps12-ycf3_p2	TAAGAGCGGGGGATTTTGTG	ATAGCACTTACTCCCGGGAA	50.0	50.0	57.9	57.8	438-1971 bp	1.0	88.2
rps12-ycf3_p3	TAAGAGCGGGGGATTTTGTG	TAGCACTTACTCCCGGGAAT	50.0	50.0	57.9	57.8	437-1970 bp	1.0	88.2
rps12-ycf3_p4	GAGGGCATCTCTTAAGAGCG	ATAGCACTTACTCCCGGGAA	55.0	50.0	57.9	57.8	450-1983 bp	1.0	88.0
rps12-ycf3_p5	GAGGGCATCTCTTAAGAGCG	TAGCACTTACTCCCGGGAAT	55.0	50.0	57.9	57.8	449-1982 bp	1.0	88.0