Hotspot

ndhF-rpl32

Genome · 142845-144744 · 1900 bp

Back to Adenophora

Pi 0.0787

MarkerSeek score 79.80

Primer available yes

Label ndhF-rpl32

Feature Metadata

label_name: ndhF-rpl32
feature_type: igs
region: Genome
length_bp: 1900
variable_sites: 501
pi: 0.0787
unique_haplotype_count: 9
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 79.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF-rpl32_p1	AAAGAAGAAGCCCTACCCCT	AGCGTGTCTACCGATTTCAC	50.0	50.0	57.9	58.0	2733-2787 bp	0.2	59.4
ndhF-rpl32_p2	AAAGAAGAAGCCCTACCCCT	CAGCGTGTCTACCGATTTCA	50.0	50.0	57.9	58.0	2734-2788 bp	0.2	59.4
ndhF-rpl32_p3	CTTTTTGTTGCGGCTGGAAA	AGCGTGTCTACCGATTTCAC	45.0	50.0	58.1	58.0	2752-2806 bp	0.2	59.4
ndhF-rpl32_p4	TTTGTTGCGGCTGGAAAAAG	AGCGTGTCTACCGATTTCAC	45.0	50.0	58.1	58.0	2749-2803 bp	0.2	59.4
ndhF-rpl32_p5	TTTGTTGCGGCTGGAAAAAG	CAGCGTGTCTACCGATTTCA	45.0	50.0	58.1	58.0	2750-2804 bp	0.2	59.4