Hotspot

ycf3-rpoB

Genome · 84364-86155 · 1792 bp

Back to Adenophora

Pi 0.0868

MarkerSeek score 65.50

Primer available yes

Label ycf3-rpoB

Feature Metadata

label_name: ycf3-rpoB
feature_type: igs
region: Genome
length_bp: 1792
variable_sites: 211
pi: 0.0868
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 0.47
markerseek_score: 65.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf3-rpoB_p1	ATAGCACTTACTCCCGGGAA	CCCTCATTTTGATCCCGGAG	50.0	55.0	57.8	58.0	753-2378 bp	1.0	90.4
ycf3-rpoB_p2	TAGCACTTACTCCCGGGAAT	CCCTCATTTTGATCCCGGAG	50.0	55.0	57.8	58.0	752-2377 bp	1.0	90.4
ycf3-rpoB_p3	ATAGCACTTACTCCCGGGAA	CTCATTTTGATCCCGGAGCA	50.0	50.0	57.8	58.0	751-2376 bp	1.0	90.3
ycf3-rpoB_p4	TAGCACTTACTCCCGGGAAT	CTCATTTTGATCCCGGAGCA	50.0	50.0	57.8	58.0	750-2375 bp	1.0	90.3
ycf3-rpoB_p5	TAGCACTTACTCCCGGGAAT	TCCCTCATTTTGATCCCGGA	50.0	50.0	57.8	58.4	753-2378 bp	1.0	87.4