Hotspot

psbJ-ycf3

Genome · 79949-82340 · 2392 bp

Back to Adenophora

Pi 0.0932

MarkerSeek score 81.50

Primer available yes

Label psbJ-ycf3

Feature Metadata

label_name: psbJ-ycf3
feature_type: igs
region: Genome
length_bp: 2392
variable_sites: 805
pi: 0.0932
unique_haplotype_count: 10
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 81.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbJ-ycf3_p1	AAGTGTGGGAAATGGCCAAT	AAAATTTCCATTGCTCCGCG	45.0	45.0	58.0	58.0	2556-2560 bp	0.2	52.0
psbJ-ycf3_p2	AAACGCTAAATTGAAGCCGC	AAAATTTCCATTGCTCCGCG	45.0	45.0	58.0	58.0	2902-2903 bp	0.2	51.7
psbJ-ycf3_p3	TCCTTCCTTAGGGAACTGGG	AAAATTTCCATTGCTCCGCG	55.0	45.0	58.0	58.0	2874-2875 bp	0.2	51.7
psbJ-ycf3_p4	TTGGGCTATGAGCAATGTCC	AAAATTTCCATTGCTCCGCG	50.0	45.0	57.9	58.0	2808-2809 bp	0.2	51.6
psbJ-ycf3_p5	CCGCTTTTTGAGTCCTTCCT	AAAATTTCCATTGCTCCGCG	50.0	45.0	58.1	58.0	2886-2887 bp	0.2	51.1